Recovery and Identification of Viable Bacteria Immured in Glacial Ice
نویسندگان
چکیده
An extraction system has been constructed that melts ice from the interior of ice cores and collects the resulting water aseptically. Using this system, bacteria entrapped in ice cores from different geographic locations, that range in age from 5 to 20,000 years old, have been isolated and characterized. Ice cores from the Guliya ice cap on the Tibetan Plateau (China) contained the highest number of colony-forming units per milliliter (∼180 cfu ml−1) and representatives of many different bacterial species. Much lower numbers of bacteria (>20 cfu ml−1) were recovered from Sajama (Bolivia) ice cores, although in general such nonpolar ice cores contained more culturable bacteria than samples of polar ice, presumably due to the closer proximity of major biological ecosystems. More bacteria were recovered from Late Holocene ice from the Taylor Dome region than from ice of the same age from the Antarctic peninsula or from Greenland. Bacterial isolates were identified, in terms of their closest phylogenetic relatives, by determining small-subunit ribosomal RNA-encoding DNA sequences (16S rDNAs), and most were related to spore-forming Bacillus and Actinomycetes species, or to nonsporulating Gram positive bacteria. The numbers of recoverable bacteria did not correlate directly with the age of the ice, indicating that most bacteria were deposited episodically in snowflakes and/or attached to larger particles of inorganic and organic debris. By identifying the features that facilitate microbial survival within terrestrial ice, extrapolations to the likelihood of microorganisms surviving frozen in water ice on Mars, Europa, or within comets will be improved. c © 2000 Academic Press
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